The aim of the present study was to examine the effects of fetal bovine serum (FBS) concentration equilibration time and oocyte pre-treatment with cytochalasin B (CCB) on subsequent development of vitrified-warmed ovine immature (GV COCs) and matured (MII) oocytes with (MII COCs) or without cumulus cells (MII DOs) In Experiment 1 the effects of FBS concentrations (10 and 20%) during the Beside cooling/warming rates and composition of vitrification solution developmental stage of immature oocytes may also affect their vitrification outcome The aim of the present study was to evaluate the selection effect of developmentally competent immature bovine oocytes by Brilliant Cresyl Blue (BCB) on maturity of oocytes after vitrification

Porcine oocyte vitrification in optimized low toxicity

Use of both vitrification solutions showed a characteristic plasticized surface In the second experiment the relative cytotoxicity of vitrification solutions (VM3 and VM3 optimized) was studied Oocyte viability chromosomal organization and the cortical granules distribution were assessed by fluorescent stain

This study was conducted to investigate the effect of vitrification on the dynamics of the global transcriptome in bovine germinal vesicle (GV) oocytes and their in vitro-derived metaphase II (MII) oocytes The GV oocytes were vitrified using the open-pulled straw method After warming GV oocytes and the resulting MII-stage oocytes were cultured in vitro for 2 h and 24 h respectively and were

Introduction The production of bovine embryos in vitro has become a routine procedure and thus successful cryopreservation methods are required for efficient utilization of these embryos (Leibo and Loskutoff 1993) In the past decade various new methods for embryo cryopreservation have been developed Among these methods vitrification has been widely used and is now regarded as a

RESEARCH ARTICLE Open Access Sequential analysis of global gene expression profiles in immature and in vitro matured bovine oocytes: potential molecular markers of oocyte maturation Solomon Mamo1* Fiona Carter1 Patrick Lonergan1 Cludia LV Leal1 2 Abdullah Al Naib1 Paul McGettigan1 Jai P Mehta1 Alexander CO Evans1 Trudee Fair1 Abstract

May 01 2013Vitrification often causes severe cryoinjury and malfunctions in oocytes e g disruption of the meiotic spindle and the cytoskeleton and damage to the mitochondria Based on IM staining by the lipophilic fluorescent dye DiO the types of IM organization within unfrozen bovine GV and MII oocytes were categorized () because dynamic characteristics of IM structure in bovine oocytes during

Nuclear maturation of immature bovine oocytes after

To date at least two well known methods have been widely used for vitrification of oocytes and embryos at different stages in a variety of species However there is no reported data regarding the comparative effectiveness of these two methods for vitrification of immature bovine oocytes The objective of this study is to compare the nuclear maturation of immature bovine oocytes vitrified

CiteSeerX - Document Details (Isaac Councill Lee Giles Pradeep Teregowda): oocytes Veterinary World 6(10): 730-733 Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15 % ethylene glycol (EG) + 15 % dimethyl

The effectiveness of different cryodevices (open-pulled straw) (OPS) electron microscopy grid (EMG) and Cryotop was evaluated for vitrification of immature bovine oocytes Polar body metaphase II stage (MII) survivability and subsequent developmental rates were determined Only oocytes with four or five layers of cumulus cells were used

The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS) Electron Microscopy Grid (EMG) and cryotop) for vitrification of immature bovine oocytes Polar body MII stage survivability and subsequent developmental rates were compared Only oocytes with 4-5 layers of cumulus cells were used

Vitrification of Immature and Mature Bovine Oocytes Paige T Hardin This Thesis is brought to you for free and open access by the Graduate School at LSU Digital Commons It has been accepted for inclusion in LSU development of this research His willingness to give

Introduction Since the first demonstration that mouse oocytes can be cryopreserved [] many workers have attempted to freeze mammalian follicular oocytes in a number of species (i e mice [] rats [] cattle [4 5] and horses []) Cryopreserved immature bovine oocytes have a lower maturation rate than frozen-thawed matured oocytes [] Several studies have demonstrated that immature oocytes

CiteSeerX - Document Details (Isaac Councill Lee Giles Pradeep Teregowda): oocytes Veterinary World 6(10): 730-733 Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15 % ethylene glycol (EG) + 15 % dimethyl

The purpose of this study was to determine the efficacy of pre‐treating mature bovine oocytes with Taxol before vitrification by the open pulled Straw method (OPS) We evaluated the effects of pre‐treating the oocytes with 1 M Taxol on chromosome organization spindle morphology cortical granule distribution and the ability of fertilized

Nuclear maturation of immature bovine oocytes after

However there is no reported data regarding the comparative effectiveness of these two methods for vitrification of immature bovine oocytes The objective of this study is to compare the nuclear maturation of immature bovine oocytes vitrified using open pulled straw (OPS) and cryotop methods Two experiments were conducted in this study

Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported the degree of cryoinjury after the vitrification has not yet been fully examined Since cryoinjury after vitrification of ovaries has been reported in bovine and humans this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL

Hurtt A Landim-Alvarenga F Scidel G Squires E Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol ficoll and sucrose solution using open-pulled straws Theriogenology 2000 54(1):119-28 CrossRef

In another study in which immature bovine oocytes were vitrified using a mixture 2 5M EG ficoll and sucrose in (open pulled straws) OPS a successful maturation rate of 60% was recorded Cetin et al in 2006 vitrified immature bovine oocytes 34 1% of oocytes reached the MII stage in EG group ( 27 )

Beside cooling/warming rates and composition of vitrification solution developmental stage of immature oocytes may also affect their vitrification outcome The aim of the present study was to evaluate the selection effect of developmentally competent immature bovine oocytes by Brilliant Cresyl Blue (BCB) on maturity of oocytes after vitrification

Research on bovine oocytes cryopreservation is important for successful preservation of genetically valuable animal In vitro fertilization and development of immature and mature bovine oocytes cryopreserved by ethylene glycol with sucrose Cryobiology Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova

Vitrification of immature bovine oocytes using glass vitrification micropipettes under normal or super-cooled LN 2 Vitrification and thawing: Two vitrification solutions resulted in viable blastocysts and live calves following were prepared in media consisting of TCM-199 with in vitro embryo production [3] Although oocyte 10% FBS

The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovine immature cumulus oocyte complexes (COCs) Bovine ovaries were collected from the local slaughterhouse in a container with 30-37C physiologic saline and supplemented with antibiotics The grade A aspirated COCs were assigned to 6 experimental groups The 1st group was control (n=100)

Apr 01 2016The objectives of this study were to compare the effectiveness of liquid helium (LHe) and liquid nitrogen (LN 2) as cryogenic liquid for vitrification of bovine immature oocytes with open-pulled straw (OPS) system and determine the optimal cryoprotectant concentration of LHe vitrification Cumulus oocyte complexes were divided into three groups namely untreated group (control) LN 2 vitrified

Jul 22 2015The aim of this study was to assess in vitro meiosis resumption and nuclear maturation of Rattus norvegicus oocytes after vitrification with different cryoprotective solutions Cumulus-oocyte complexes (COCs) were exposed to an equilibration solution for 4 min placed in cryoprotective solutions for 1 min and vitrified in open pulled straws

The aim of the present study was to examine the effects of fetal bovine serum (FBS) concentration equilibration time and oocyte pre-treatment with cytochalasin B (CCB) on subsequent development of vitrified-warmed ovine immature (GV COCs) and matured (MII) oocytes with (MII COCs) or without cumulus cells (MII DOs) In Experiment 1 the effects of FBS concentrations (10 and 20%) during the

open The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis These results suggest that immature

Jun 01 2005Abstract To improve the feasibility of nylon-mesh holder for vitrification of bovine cumulus-oocytes complexes (GV-COCs) having germinal vesicle this study was conducted to demonstrate effects of sugars and protocol of exposure in vitrification on subsequent in vitro maturation ultrastructural changes and in vitro development in bovine immature oocytes after cryopreservation